CloneID: E104
Antigen Long Description: The original antibody was raised by immunizing a C57BL/6 mouse with DENV-2.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P29990
Specificity Statement: This antibody is specific for DIII C-C′ loop of Envelope protein E of Dengue virus type 2
Application Notes (Clone): The original antibody was used for a plaque reduction assay on DENV-2 (Zhang et al., 2015; PMID:25355881, Sukupolvi-Petty et al., 2010; PMID:20592088). The Fab was incubated with DENV-2 and then added to BHK21 cells. The structure of the original antibody was determined using cryo-EM (Zhang et al., 2015; PMID:25355881). The original version of this antibody was used for flow cytometry on DENV infected cells. This antibody was used for an in vivo protection assay on BALB/c mice. Here the mice were given the antibody after infection with the DENV-2. It showed improvement over control (Sukupolvi-Petty et al., 2010; PMID:20592088). This antibody was used for an ELISA on immature virus particles. This antibody was able to promote infectivity of DENV-2. In short it made normally uninfectious immature virus infectious (da Silva Voorham et al., 2012; PMID:22431958).
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