CloneID: MA28-7
Antigen Long Description: The original antibody was raised by immunizing BALB/c mice with formalin-inactivated EV71 strain 1095.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: Q66479
Specificity Statement: This antibody is specific for binds specifically to the 5-fold vertex encompassing VP1-98E and VP1-244K.
Application Notes (Clone): The original version of this antibody was used for a neutralization assay by preincubating the EV71 (Human enterovirus 71) virus with the antibody and then adding Vero cells. This antibody neutralized only EV71 strains B4, B3 and C2. The structure of the fab version of this antibody was determined using cryo-EM (Lee et al., 2013; PMID:23946455). This antibody was used to immunoprecipitate EV-A71-1095 capsids from solution (Nishimura et al., 2015; PMID:26430888).
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