CloneID: 2D3
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was generated by immunizing 6-8 weeks old female BALB/c mice with his-tagged human GITR protein.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: Q9Y5U5
Specificity Statement: This antibody binds human and rhesus monkey GITR (TNFRSF18; CD357) and does not cross react with mouse GITR. This protein acts as a receptor for TNFSF18, which is involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death.
Application Notes (Clone): The initial binding characterization of the antibody was done using ELISA. The binding reactivity of this antibody to human, mouse and rhesus monkey GITR was determined using flow cytometry. This antibody was shown to cross react with human and rhesus monkey GITR and not mouse GITR. The binding affinity of the original mouse IgG1 antibody for human GITR was determined using surface plasmon resonance and was found to be Kd= 7.75X 10^(-10)M. The anti-tumor effect of this antibody was tested in vivo in a mouse colon cancer model. Murine MC-38 cells (colon adenocarcinoma cells) were injected subcutaneously in B-hGITR mice and treated with this antibody. It was reported that this antibody showed good tumor inhibitory effect (CN111763257).
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