CloneID: 1G8
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was generated by immunizing BALB/c mice with synthetic N-terminal glycopeptide (VHLTPEC) and KLH conjugate.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This is a chimeric Fc Silent™ reformatted human IgG1 antibody, based on the original mouse IgG2a format, created for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P68871
Specificity Statement: This antibody binds human hemoglobin glycated at the N-terminal valine of the hemoglobin β chain and does not cross react with non-glycated hemoglobin. Glycated hemoglobin is formed by the conjugation of a hexose sugar to the beta-chain of hemoglobin. Glycosylated hemoglobin A1c (HbA1c) levels are used as an indicator of average plasma glucose concentration in diabetic patients. Levels of more than 48 mmol/mol (6.5%) are used as a criterion for the diagnosis of diabetes. Higher levels of HbA1c are also associated with cardiovascular disease.
Application Notes (Clone): This antibody is recommended for detection of HbA1c levels in plasma and blood samples. The binding reactivity of this antibody towards HbA1c and non-glycated hemoglobin was done using indirect ELISA. The binding reactivity of this antibody to HbA1c was compared to commercially available anti-HbA1c antibody BM300-F7C2 and it was reported that this antibody showed better specificity for HbA1c (CN110724671).
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