CloneID: anti-CTP3-TE33
Antigen Long Description: The original antibody was raised by immunizing a Balb/c mouse with amino acid residues 50-64 of the B subunit of cholera toxin coupled to tetanus toxoid
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P32890; Q57193
Specificity Statement: The epitope of this antibody is the sequence: VEVPGSQHIDSQKKA from Cholera toxin peptide 3 (CTP3)
Application Notes (Clone): The original antibody was used for fluorescence quenching on DNP-CTP3. This experiment was performed to determine the binding affinity of this antibody, which is 1.2 x 10^6 M^-1. The antibody was used for a radioimmunoassay on cholera toxin and H-LT, a heat-labile toxin produced by enterotoxigenic strains of E. coli isolated from humans. The antibody bound to both antigens. The antibody was used for a western blot on cholera toxin and H-LT, a heat-labile toxin produced by enterotoxigenic strains of E. coli isolated from humans. Which showed the same results as the radioimmunoassay (Anglister et al., 1988; PMID:2450576). The structure of this antibody was determined using NMR measurements (Zilber et al., 1990; PMID:2271636). The structure of this antibody was determined using X-ray crystallography (Shoham, 1993; PMID:7690406). This antibody was used for Phage-colony dot immunoblotting. This antibody was used for an ELISA on synthetic peptide VEVPGSQHIDSQKKA. Further the antibody was used for surface plasmon resonance on the same peptide (Otte et al., 2006; PMID:16273596).
English
Deutsch
