CloneID: HA1
Heavy Chain modification: Fc Silent™
Antigen Long Description: Generated mAbs to HAV by phage display of the antibody library generated from the peripheral blood lymphocytes of immune donor recovered from hepatitis A. The cDNAs encoding the immunoglobulin gamma1 Fd and kappa light chains were synthesized from total RNA of the PBLs of the HAV-immune donors and cloned into a phage display vector to construct a combinatorial library of clones. The phage-displayed Fab library was panned four times against HAV. Analysis of the antigen-binding activities of the pooled phages after each round of the panning indicated that HAV-binding phage clones were almost selected after the third round of panning. Finally, 24 phage clones after the fourth panning were selected, and the gene III fragment was removed to produce soluble Fab. Analysis of the antigen-binding activities of the 24 Fab clones by ELISA revealed that 22 clones were positive in HAV binding, which could be grouped into 4 different clones; HA1, HA6, HA9, and HA12.
Buffer Composition: PBS only.
Specificity Statement: Reactive to both 14S and 70S HAV particles. HA1 and HA12 recognise distinct epitopes, whereas HA6 recognises an epitope that overlaps with that of HA1 or HA12. Competitive binding assays have shown that all of these mAbs overlap with the epitopes bound by murine mAb in a single immunodominant neutralisation site on the HAV capsid.
Application Notes (Clone): HA1 has been shown to have HAV-neutralising activity through its use in radioimmunofocus inhibition assays (Kim 2003).
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