CloneID: 1A3
Antigen Long Description: The original antibody was generated by immunizing Balb/c mice with recombinant human HGF.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P14210
Specificity Statement: The antibody is specific for human HGF.
Application Notes (Clone): The specificity of the original format of the antibody was confirmed by ELISA analysis (IC50 = 5.65 nM). The binding affinity of the original format of the antibody to human HGF was measured by surface plasmon resonance (Kd= 30.1 pM). The antibody could bind to non-reduced and reduced rhHGF by western blot analysis. The antibody could efficiently neutralize HGF binding to c-Met. The antibody inhibited HGF induced proliferation in 4MBr-5 cells. The ability of the original format of the antibody to inhibit tumor growth was tested in an U87MG xenograft model. The percent of inhibition of the antibody was measured to be 73%. The ability of the original format of the antibody to inhibit tumor growth was tested in an U118 xenograft model. The percent of inhibition of the antibody was measured to be 57% (US7659378B2).
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