CloneID: 874F7-Hu1
Antigen Long Description: The original mouse antibody was generated by immunizing BALB/c mice intraperitoneally with recombinantly expressed human IL-33-his tagged protein. The humanized version was generated by grafting CDRs of the original mouse antibody onto human framework regions.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: O95760
Specificity Statement: This antibody binds an epitope between amino acid residues 41-70 of the human IL-33. This antibody can also cross react with mouse and cynomolgus monkey IL-33. Interleukin 33 (IL-33) is a multifunctional cytokine which is constitutively expressed in structural cells such as smooth muscle cells, fibroblasts, mast cells, dendritic cells, macrophages, osteoblasts, epithelial cells, and endothelial cells. It is involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines like IL-4, IL-5 and IL-13. It is also involved in activation of mast cells, basophils, eosinophils and natural killer cells. A study found that IL-33 is a bifunctional protein. On the one hand, IL-33 is localized in the nucleus and acts as a transcription factor; on the other hand, IL-33 is secreted outside the cell and acts as a cytokine by interacting with its receptor ST2.
Application Notes (Clone): The binding characterization and determination of cross-reactivity of this antibody to human, mouse and cynomolgus monkey IL-33 was done using ELISA. This antibody binds human, mouse and cynomolgus monkey IL-33 with a binding affinity of Kd=1.13 nM, Kd= 0.332 nM and Kd=1.12 nM respectively. This antibody can block the binding of IL-33 to its receptor ST2 with an IC50 of 0.451 nM. This antibody can effectively inhibit IL-33 induced 16-6 expression in HUVEC cells, IL-5 and IL-13 secretion by KU812 cells. This antibody also showed inhibitory effect on IL-33 induced PBMC and NK cells secretion of IFN gamma. This antibody was reported to effectively inhibit mouse peripheral blood IL-15 secretion in vivo. This antibody could also inhibit the number of eosinophils stimulated by human IL-33 in the peripheral blood of mice. The in vivo pharmacodynamic activity of this antibody was studied using spleen weighing assay (WO2022063281).
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