CloneID: FAb1.1
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was generated by panning a Fab library against IL-34.
Buffer Composition: PBS only.
Chimeric Use Statement: This is a reformatted human IgG1 Fc Silent™ antibody, based on the original human Fab format, created for improved compatibility with existing reagents, assays and techniques.
Uniprot Accession No.: Q6ZMJ4
Specificity Statement: The antibody is specific for human and murine IL-34. The antibody recognizes a largely continuous area at the junction of two IL-34 protomers. The epitope overlaps largely with binding epitopes responsible for CSF-1R binding.
Application Notes (Clone): The crystal structure of the Fab fragment of the antibody in complex with human IL-34 was determined. The original antibody was reformatted as an IgG molecules and the binding affinity of both formats measured by BLI experiments (Kd= 17 nM and 120 pM respectively). The antibody blocked the biological activity of IL-34 in human monocyte viability. The antibody neutralized IL-34 in a proliferation assays (Ma et al., 2012; PMID: 22483114).
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