CloneID: VACC-E7
Antigen Long Description: Two llamas were immunized with a combination of killed vaccinia virus material and purified recombinant L1 antigen. A VHH library was then constructed and panned against killed vaccinia virus .
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: The antibody is specific for vaccinia virus. The antibody does not recognize the L1 antigen. Vaccinia virus is a large, complex, enveloped virus belonging to the poxvirus family.
Application Notes (Clone): The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody was assayed as both capture and tracer in MAGPIX bead-based sandwich assays for the detection of vaccinia virus and a limit of detection (LOD) of 4x10^5 pfu/ml was achieved (Walper et al., 2014; PMID: 25211488).