CloneID: A6
Heavy Chain modification: His-Tagged
Antigen Long Description: The original antibody was raised by immunizing a Balb/c mouse with Membrane-enriched fractions of ~5x 10^10 human Raji cells affinity purified on rhuIFN-y coupled to Affigel-10.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P15260
Specificity Statement: This antibody is specific for Interferon gamma receptor 1.
Application Notes (Clone): The original antibody was used for a radioimmunoassay on 125I-labeled rhuIFN-gamma (Aguet et al., 1987; PMID:2435837, Bridges et al., PMID:8738212). This antibody was able to immunoprecipitate huIFN-gamma from lysates of enriched Raji cell membranes (Aguet et al., 1987; PMID:2435837). The structure of the original antibody was determined using x-ray crystallography (Sogabe et al., 1997; PMID:9367779, Hofstädter et al., 1999; PMID:9878445). The scFv version of this antibody was used for surface plasmon resonance on intact IFNyR (Bridges et al., PMID:8738212, Hofstädter et al., 1999; PMID:9878445).