CloneID: KE9
Antigen Long Description: This antibody was raised panning against a library created by immunizing llamas with either breast cancer biopsy lysates.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original VHH format for improved compatibility with existing reagents assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P08727
Specificity Statement: This antibody is specific for human KRT19. KRT19 is involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Application Notes (Clone): To help identify the target of this antibody immunoprecipitation was preformed on T47D cells using the VHH version of this antibody bound to streptavidin beads. Further the ability of this antibody to be used for breast cancer research was assayed using immunohistochemistry. In short, breast cancer tissue was stained using the VHH version of this antibody. Finally to characterize this antibody. An ELISA was preformed using the VHH version of this antibody on KRT19 (Even-Desrumeaux et al, 2012; pmid22772166).
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