CloneID: YBM15.1
Antigen Long Description: This antibody was derived from fusions between the 210 RC Y3/Ag 1.2.3 rat myeloma cell line and spleen cells from rats hyperimmunized with adult mouse bone marrow.
Buffer Composition: PBS only.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Uniprot Accession No.: P05555
Specificity Statement: This antibody recognizes murine integrine MAC-1 (Itgam/Cd11b). This integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens.
Application Notes (Clone): This antibody is recommended for the detection and analysis of MAC-1, the murine equivalent of CD11b. For instance, YBM15.1 was used in a panel of antibodies in the assessment of the presence of antigenic determinants on normal erythroid progenitors via flow cytometry (Watt et al., 1987; pmid: 3330997). This antibody was used to detect the integrin MAC-1 in the neural tissue of Biozzi ABH mice infected with Semliki Forest virus (SFV) A7(74) via immunohistochemistry (Smith et al., 2000; pmid: 10814783).
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