CloneID: 7A2
Heavy Chain modification: ScFv
Antigen Long Description: The original antibody was selected by biopanning with a scFv phage library performed on solid-phase recombinant MET928.
Buffer Composition: PBS only.
Chimeric Use Statement: This is a reformatted mouse scFv antibody, based on the original mouse scFv format, created for improved compatibility with existing reagents, assays and techniques.
Uniprot Accession No.: P08581
Specificity Statement: The antibody specifically binds to amino acid residues 568-741 of human MET.
Application Notes (Clone): The scFv fragment showed inhibition of HGF/SF induced migration of SKOV cells. In particular, the scFv fragment had an IC50 of 2.3 nM in the SKOV migration assay. The binding affinity of the scFv fragment to MET928 was measured by surface plasmon resonance (Kd= 3.6 nM). In a competition ELISA, binding of 2.5 nM of the Fab version of the antibody to a MET928-coated surface was inhibited by 15 μM and 5 μM NK1 (US20150147274A1).
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