CloneID: ham151-149
Antigen Long Description: The original antibody was generated from a hamster immunized with a complex of mouse Bim and Bcl-XL produced in insect cells.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Hamster IgG format for improved compatibility with existing reagents assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: O54918
Specificity Statement: The antibody reacts with mouse Bim. Mouse Bim is a 19-24 kDa pro-apoptotic protein (Bcl-2 family) which regulates immunological responses.
Application Notes (Clone): The antibody reacted in Western blots and by immunofluorescence with permeabilized B and T cells from Bim +/+ mice (Oliver et al., 2004; PMID: 15520248). T cells were analyzed for levels of Bcl-2 and Bim protein by staining of permeabilized cells with the antibody (Jorgensen et al., 2007; PMID: 17785775). The antibody was used for flow cytometry analysis (Kashiwakura et al., 2013; PMID: 23278598).
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