Anti-N-ethylmaleimide cysteine (OX-133)

Anti-N-ethylmaleimide cysteine [OX-133], Recombinant, IgG1 lambda, Mouse

CloneID: OX-133

Antigen Long Description: Purified MBP-gp120 (HIV-1) reduced using tris(2-carboxyethyl)phosphine (TCEP, 2.5 mM) for 20 minutes and alkylated using NEM (5 mM solubilized in PBS) for 30 minutes.

Buffer Composition: PBS only.

Specificity Statement: This antibody recognises cysteine-boud N-ethylmaleimide in a sequence-independent manner. It has no affinity for unmodified peptides or peptides modified with other alkylating agents such as iodoacetamide.

Application Notes (Clone): OX133 detects NEM-modified proteins and can be used as an affinity matrix to purify NEM-modified proteins from cell lysates. OX133 does not cross react with any other alkylating agent, making it a highly selective reagent for the purification of NEM-labeled proteins and potentially peptides for mass spectrometry-based analysis.

• SKU: ABAAb00579-1.1-BT
• Manufacturer: Absolute Antibody
• Manufacturer SKU: Ab00579-1.1-BT
• Package Unit: 1 mg
• Quantity Unit: STK
• Reactivity: Various species
• Clonality: Recombinant
• Application: Immunoprecipitation, ELISA, Flow Cytometry
• Isotype: IgG1 lambda
• Host: Mouse