Anti-PD-1 (1G4)

Anti-PD-1 [1G4], Recombinant, IgG4 kappa, Human

CloneID: 1G4

Antigen Long Description: The original antibody was generated by immunizing 6-8 weeks old female BALB/c mice with his-tagged human PD-1 protein.

Buffer Composition: PBS only.

Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG4 format for improved compatibility with existing reagents assays and techniques.

Uniprot Accession No.: Q15116

Specificity Statement: This antibody binds human PD-1 protein. PD-1 acts as an inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. It delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2.

Application Notes (Clone): The initial binding characterization of the antibody was done using ELISA. The binding reactivity of this antibody to human, mouse and rhesus monkey PD-1 expressed on CHO cells was determined using flow cytometry. This antibody was shown to cross react with human and rhesus monkey PD-1 and not mouse PD-1. The binding affinity of the original mouse IgG4 antibody for human PD-1 was determined using surface plasmon resonance and was found to be Kd= 1.07 nM. The anti-tumor effect of this antibody was tested in vivo in a mouse colon cancer model. Murine MC-38 cells (colon adenocarcinoma cells) were injected subcutaneously in B-hPD-1 mice and treated with this antibody. It was reported that this antibody showed good tumor inhibitory effect (CN111763257).

• SKU: ABAAb03364-13.0-BT
• Manufacturer: Absolute Antibody
• Manufacturer SKU: Ab03364-13.0-BT
• Package Unit: 1 mg
• Quantity Unit: STK
• Reactivity: Human, Rhesus Monkey (Primate)
• Clonality: Recombinant
• Application: ELISA, Flow Cytometry
• Isotype: IgG4 kappa
• Host: Human