CloneID: 10F.9G2
Heavy Chain modification: Fc Silent™
Antigen Long Description: This antibody was raised by immunising Lewis rats with murine PD-L1 cDNA and murine PD-L1 CHO transfectants.
Buffer Composition: PBS only.
Chimeric Use Statement: This is a bispecific, knob-into-hole heterodimerized antibody, with one arm directed against mouse PD-L1 (Fab) and one arm directed against mouse CD28 (scFv based on the superagonistic clone D665).
Uniprot Accession No.: Q9EP73
Specificity Statement: This antibody is specific for murine PD-L1.
Application Notes (Clone): The specificity of this antibody has been confirmed in ELISA analysis (Eppihimer et al, 2002). This antibody reacts specifically with mPD-L1-transfected cells in flow cytometry, and has been used to assess microvascular endothelial cells PD-L1 expression (Eppihimer et al, 2002). When administered to mice, a radiolabelled version of this antibody has been used to quantify PD-L1 expression in vivo, by measuring antibody accumulation in different tissues (Eppihimer et al, 2002). This antibody has also been used in immunohistochemical analysis of PD-L1 expression in frozen tissue sections of murine brain (Eppihimer et al, 2002) and murine hearts (Rodig et al, 2003). This antibody has been shown to block the binding of PD-L1 to both PD-1 and B7-1 (Paterson et al, 2011). This antibody precipitates diabetes in NOD mice and in adoptive transfer models of CD4+ and CD8+ T cell-driven diabetes, and accelerates diabetes in recipients of T cells from diabetic and prediabetic mice (Paterson et al, 2011).
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