CloneID: 29E.2A3
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: Q9NZQ7
Specificity Statement: This antibody binds human PD-L1.
Application Notes (Clone): The original format of the antibody was able to block the interaction between PD-L1 on the 300.19-hPD-L1 cells and B7-1 on the plate and the interaction between PD-L1 on the 300.19-hPD-L1 cells and PD-1 on the plate (Butte et al., 2008; PMID: 18585785). This antibody was used for detection of PD-L1 expressed in 293T cells by flow cytometry (Broos et al. 2019, PMID: 31394834). The humanized version of the antibody was constructed (AU2019201688). Immunohistochemical staining of frozen tissue sections was performed using the antibody to stain thymic epithelia (Brown et al., 2003; PMID: 12538684).
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