Anti-PD-L1 (29E.2A3)

Anti-PD-L1 [29E.2A3], Recombinant, IgG kappa, Rabbit

CloneID: 29E.2A3

Buffer Composition: PBS only.

Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.

Uniprot Accession No.: Q9NZQ7

Specificity Statement: This antibody binds human PD-L1.

Application Notes (Clone): The original format of the antibody was able to block the interaction between PD-L1 on the 300.19-hPD-L1 cells and B7-1 on the plate and the interaction between PD-L1 on the 300.19-hPD-L1 cells and PD-1 on the plate (Butte et al., 2008; PMID: 18585785). This antibody was used for detection of PD-L1 expressed in 293T cells by flow cytometry (Broos et al. 2019, PMID: 31394834). The humanized version of the antibody was constructed (AU2019201688). Immunohistochemical staining of frozen tissue sections was performed using the antibody to stain thymic epithelia (Brown et al., 2003; PMID: 12538684).

• SKU: ABAAb03452-23.0-BT
• Manufacturer: Absolute Antibody
• Manufacturer SKU: Ab03452-23.0-BT
• Package Unit: 1 mg
• Quantity Unit: STK
• Reactivity: Human
• Clonality: Recombinant
• Application: Flow Cytometry, Immunohistochemistry, Blocking
• Isotype: IgG kappa
• Host: Rabbit