CloneID: B9
Antigen Long Description: The original antibody was generated by immunizing an alpaca with recombinant Pf12p protein and constructing a phage library. The antibody was isolated by biopanning against Pf12p.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This is a chimeric antibody, developed from the original camelid VHH antibody, specifically engineered for enhanced compatibility with existing reagents, assays, and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: C6KSX1
Specificity Statement: The antibody recognizes Pf12p. The antibody does not cross-react with Pf12 or Pf41. The antibody binds specifically to the Pf12p interdomain (D1-D2 junction) area.
Application Notes (Clone): The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody recognised reduced and non-reduced Pf12p in western blot analysis. The binding affinity of the original format of the antibody to Pf12p was measured by surface plasmon resonance (Kd= 6.7 nM). The structure of the antibody in complex with recombinant Pf12p was determined by X-ray crystallography (Dietrich et al., 2021; PMID: 33480416).
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