CloneID: 14B3
Antigen Long Description: The original antibody was generated by immunizing rabbits with PR protein.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Rabbit IgG format for improved compatibility with existing reagents assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P06401
Specificity Statement: The antibody is specific for PR protein. PR is a protein molecule, a cellular core receptor belonging to a steroidal superfamily, which can be bonded to the corresponding hormone to form a hormone-receptor complex, enabling hormones to exert their biological effects.
Application Notes (Clone): The specificity of the original format of the antibody was confirmed by ELISA analysis (affinity constant of 1.82 × 10^10 M-1). The antibody specifically detected PR protein in Western blot analysis. The antibody 14B3 and the commercial antibody 1E2 were used to stain breast cancer and endometrial. Results showed the antibody specificity is comparable to the commercial antibody. Further, the sensitivity of the antibody 14B3 was higher than the commercial antibody (CN113583132A).
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