CloneID: A10B
Heavy Chain modification: His-Tagged
Antigen Long Description: The antibody was originally generated by immunizing BALB/c mice with purified rabbit IgG.
Buffer Composition: PBS only.
Specificity Statement: The antibody recognizes the constant heavy chain 1 (CH1) domain of rabbit IgG.
Application Notes (Clone): Different formats of the antibody (scFv-cys, scFv, Fab and IgG) were employed for the construction of biosensors used for antigen detection in complex samples. In scFv-cys, one glycine of the linker was replaced with a cysteine, which determine an orientation of the scFv fragments on the sensor surface. The performance, in terms of sensitivity and selectivity, in biosensors prepared with scFv-cys, scFv, Fab and IgG was compared. The scFv-cys based biosensors displayed greater assay sensitivity and exhibited less nonspecific activity than intact monoclonal IgG and Fab fragment-based immunosensors. The scFv-cys biosensor surface was easily regenerated by stripping the rabbit IgG from the surface using an acidic solution. Moreover, western blot analysis showed purified scFv migrated as a single band at a MW of ~32 000 and did not appear to be degraded and the specificity of IgG1, Fab fragment and scFv towards rabbit IgG was determined by ELISA. (PMID: 15679346) In another study, two histidine amino acids were incorporated into the linker peptide of the scFv fragment and the scFv-His fragment was then employed to develop a new biosensor. The specificity of scFv and scFv-Hys were confirmed by ELISA. (PMID: 16255580) The affinity constant of rabbit IgG binding with scFv measured by using a conventional regeneration SPR method ((2.2 ± 1.5) × 10^7 M−1) was comparable with the value determined with a nonregeneration protocol (2.5 ± 0.2) × 10^7 M−1, and quartz crystal microbalance (1.9 × 10^7 M−1). (PMID: 16536419)
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