CloneID: AC2
Heavy Chain modification: ScFv
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This is a reformatted mouse scFv antibody, based on the original mouse scFv format, created for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: The antibody is specific for ractopamine. The antibody does not cross react with clenbuterol, mabuterol, terbutanline, adrenalin, chloramphenicol and enrofloxacin.
Application Notes (Clone): The specificity of the original format of the antibody was confirmed by ELISA analysis (CN103160515B). The single-chain variable fragment of the antibody conjugated to alkaline phosphatase (asFv-AP) fusion protein was used for the development of a rapid, sensitive chemiluminescent enzyme immunoassay (CLEIA) and direct competitive CLEIA for ractopamine (IC50= 0.25 ng/mL, LOD= 0.02 ng/mL, working detection range = 0.05–1.45 ng/mL) (Dong et al., 2012; PMID: 22769009).
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