CloneID: 1E2
Antigen Long Description: The original antibody was generated from a panel of humanized single domain antibodies derived from a synthetic library. The antibody was isolated by panning against SARS-CoV-2 spike receptor-binding domain (RBD).
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P0DTC2
Specificity Statement: The antibody binds to the RBD of the Spike protein of SARS-CoV.
Application Notes (Clone): The specificity of the original format of the antibody was confirmed by ELISA analysis. The binding affinity of the VHH fragment to SARS-CoV-2 RBD was measured by surface plasmon resonance (Kd= 35.5 nM). The VHH showed half maximal neutralization concentration (EC50) of 0.069 µg/mL and 0.2401 µg/mL against SARS-CoV-2 pseudotypes, and authentic SARS-CoV-2, respectively. Fusion of the human IgG1 Fc to the VHH improved the neutralizing activity compared to the original format of the antibody. The Fc-fused VHH demonstrated potency with EC50 of 0.538 nM. (Chi et al, 2020; pmid: 32913273)
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