CloneID: MAb4-5
Antigen Long Description: The original antibody was isolated using whole SFTSV virions as bait from a phage-display antibody library derived from the peripheral blood mononuclear cells of a patient that recovered from SFTS disease.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: R4V2Q5
Specificity Statement: The antibody is specific for SFTSV. It binds to a linear epitope in the ectodomain of glycoprotein Gn. It does not cross react with RVFV.
Application Notes (Clone): The antibody showed neutralizing activity against SFTSV infection in Vero cells (Guo et al. 2013; PMID: 23863504). To verify neutralizing activity, full-length human IgG1 antibody was constructed. The recombinant antibodies were expressed by 293T mammalian cells and the concentration required to obtain 50% neutralization of 100 TCID50 SFTSV in vitro was 44.2 μg/mL. The interaction between Gn and the antibody was further demonstrated by surface plasmon resonance (SPR) assays with a dissociation constant (Kd) of 25.9 nM. The crystal structure of SFTSV Gn head domain in complex with the Fab fragment of the antibody was determined. The antibody could recognize the denatured SFTSV Gn head by WB (Wu et al., 2017; PMID: 28827346).
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