CloneID: NbsfGFP01
Antigen Long Description: The original antibody was generated by immunizing an adult one-humped camel with recombinant superfolder green fluorescent protein (sfGFP). An immune nanobody library was generated from the peripheral blood of the immunized camel. The particular antibody was isolated by panning the library against sfGFP.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P42212
Specificity Statement: This antibody is specific for superfolder green fluorescent protein which is a better-folding GFP version, with the following mutations: S30R, Y39N, F64L, S65T, F99S, N105T, Y145F, M153T, V163A, I171V and A206V.
Application Notes (Clone): The binding specificity of this nanobody towards sfGFP was determined using ELISA. This antibody can also be used of the identification of sfGFP via a western blot (PMID: 25085037).
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