Anti-single-stranded poly(rI) RNA (Jel 103)

Anti-single-stranded poly(rI) RNA (Jel 103), Recombinant, IgG kappa, Host: Rabbit

CloneID: Jel 103

Antigen Long Description: Jel 103 was selected from a hybridoma cell line generated by the fusion of the MOPC 315-43 myeloma cell line with mouse spleen cells derived from C57/B1 mice which had been immunized with poly(rI)poly(dC) duplex complexed to methylated bovine serum albumin.

Origin Pub PMID: 7523684

Buffer Composition: PBS only.

Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Specificity Statement: Jel 103 is highly specific for single-stranded RNA and shows sequence specificity as it binds to poly(rI) but not to poly(dI), poly(rG) or to any other homopolymer - it does not bind randomly to calf thymus DNA. Jel 103 Fab is shown to form base stacking interactions with a Tyr residue and two salt-bridge interactions to the phosphate backbone with Lys and Arg residues.

Application Notes (Clone): Jel 301 can be used for the detection of single-stranded poly(rI) and for binding studies where the antibody has been used previosly in solid-phase radioimmunoassays.

• SKU: ABAAb00800-23.0-BT
• Manufacturer: Absolute Antibody
• Manufacturer SKU: Ab00800-23.0-BT
• Package Unit: 1 mg
• Quantity Unit: STK
• Reactivity: Various species
• Clonality: Recombinant
• Application: Immunofluorescence
• Isotype: IgG kappa
• Host: Rabbit