CloneID: AbAb1-pSTAT3
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was generated by immunizing rabbits with recombinant phosphorylated peptide pSTAT3 (PGSAAP-pY-LKTKGGGSC) conjugated with KLH as an immunogen.
Buffer Composition: PBS only.
Chimeric Use Statement: This is a reformatted mouse IgG2b Fc Silent™ antibody, based on the original mouse IgG format, created for improved compatibility with existing reagents, assays and techniques.
Uniprot Accession No.: P40763
Specificity Statement: This antibody binds human phosphorylated STAT3. This protein mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors.
Application Notes (Clone): The binding reactivity of this antibody to recombinant phosphorylated pSTAT3 peptide was confirmed using ELISA. The binding reactivity was further confirmed using western blot, of STAT3 protein IFN-α-treated cells. The scFv version of this antibody was used fused to human IgG1 using a suitable linker for the generation of scFv-Fc mini-antibody. This minibody was capable of immunoprecipitating pSTAT3 in the cell lysate of HeLa cells treated with IFN-α (US20090233358).
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