CloneID: 32D5
Antigen Long Description: The original antibody was generated by immunizing mice with the recombinant TdT protein.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P04053
Specificity Statement: The antibody is specific for TdT. TdT catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.
Application Notes (Clone): The antibody specifically detected TDT protein in Western blot analysis. The antibody 32D5 and the commercial antibody SEN28 were used to stain lymphoma and thymus. Results showed the antibody specificity is comparable to the commercial antibody. Further, the sensitivity of the antibody 32D5 was higher than the commercial antibody. The antibody and the commercially available antibody TA347 were simultaneously detected on the normal tissue chip, indicating that the specificity of the antibody in normal tissue was equivalent to that of the commercially available antibody (CN113265003A).
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