CloneID: 1D11.16.8
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was generated by intraperitoneally (IP) injecting female Balb/c mice with 10 ug of native bovine TGF-B2 with complete Freund's adjuvant.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This is a reformatted human IgG1 Fc Silent™ antibody, based on the original human IgG1 format, created for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P18341; P21214; A6QP91
Specificity Statement: This antibody recognizes and binds human, mouse, rat, cow, dog, hamster, monkey and other non-human primate transforming growth factor (TGF) beta 1, 2 and 3. TGF beta is a multifunctional cytokine that exists as five isoforms (TGF beta 1-5) that share 70-80% homology with each other. These proteins are produced by many cell types and function as growth and differentiation factors.
Application Notes (Clone): This antibody was able to neutralize the biological activity of both TGF-beta 1 and beta 2 in vitro. This antibody could detect the dimeric form of TGF-beta 2 in western blots. This antibody was also reported to immunoprecipitate [125I]-TGF-beta 2 (PMID: 2537357). This antibody is also able to neutralize the growth promoting actions of TGF-B1 and B2 in the NRK proliferation assay and TGF-B induced alkaline phosphatase production in an assay using the ROS 17.2.8 osteosarcoma cell line (US5571714). This antibody was used for detection of TGF-β production in vivo in spleens sections of L. donovani-infected hamsters using immunohistochemistry (PMID: 9488418). This antibody was also used for the immunohistochemical analysis of FFPE human renal biopsy specimens for presence of TGF-beta (PMID: 10515378).
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