CloneID: 133-E12
Heavy Chain modification: His-tagged
Antigen Long Description: The original antibody was generated by immunizing llamas with a cocktail of murine and human Toll-like receptors (TLRs) that contained recombinant human TLR4/MD2, recombinant human TLR3, recombinant murine TLR3, and recombinant murine TLR2/Fc chimera. Peripheral blood mononuclear cells were isolated from the animals' blood for the construction of a phage display library, and the library was panned against the TLRs used for immunization.
Buffer Composition: PBS only.
Uniprot Accession No.: O00206
Specificity Statement: This antibody is specific for human TLR4.
Application Notes (Clone): The binding specificity of the original version of this antibody (VHH) was tested using ELISA. In a cell-based neutralizing assay with hTLR4-overexpressing HEK cells, an agonistic effect was observed, as measured by increased NFκB expression after cell lysis compared to the control group, indicating activation or enhancement of TLR activity instead of neutralization (WO2009138494A2).