CloneID: rE3
Antigen Long Description: The original antibody was generated by immunizing wild-type Wister rat with Ba/F3 cell line expressing hTLR7-Flag-Hisx6/hUnc93B1-HAx2 mixed with a suitable adjuvant.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Rat IgG2a format for improved compatibility with existing reagents assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: Q9NYK1
Specificity Statement: This antibody binds human toll-like receptor 7 (TLR7). It also cross reacts with monkey TLR7. It is an endosomal receptor that plays a key role in innate and adaptive immunity. It also controls host immune response against pathogens through recognition of uridine-containing single strand RNAs (ssRNAs) of viral origin or guanosine analogs. TLR7 is considered to be a useful marker in indicating pathological condition of autoimmune diseases.
Application Notes (Clone): This antibody was used in combination with another anti-TLR7 antibody called TLM3 to detect human TLR7 in a sandwich ELISA. LTM3 was used as used as a capturing antibody and biotinylated rE3 antibody (rE3-bio) was used a detecting antibody. This antibody can be used for the detection of human TLR7 in serum or plasma derived from human peripheral blood. The binding ability of this antibody for Ba/F3 cell line expressing human TLR7, mouse TLR7 and monkey TLR7 was checked using flow cytometry. This antibody can successfully detect human and monkey TLR7, but not mouse TLR7 in a flow cytometry. This antibody was also used in the detection of soluble human TLR7 in a sample using immunoprecipitation (US20210199667).
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