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Encompassing Amino Acids: 2-403
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 µM Na orthovanadate, 0.5 mM ATP, 4 µM Aurora A substrate, and 4 µg/ml Aurora A kinase.
Description: Human Aurora kinase A (GenBank Accession No. NM_003600), a.a. 2-403 with N-terminal His-tag, MW=50 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 3 mM DTT.
Genbank: NM_003600
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: O14965
Unit Definition: One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Ser/Thr 1 peptide substrate per minute at pH 7.4 and 30°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Ouchi,M. et al., J. Biol Chem. 279 (19), 19643-19648 (2004).
2. Kunitoku, N. et al., Dev. Cell 5 (6), 853-864 (2003).
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