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Applications: Study the consequences of deficient MHC class I and II.Study T cell activation, antigen presentation, and immune responses.Useful for the development of improved universal CAR-T or other effector cells.
Background: Beta-2-Microglobulin is a required component of Major Histocompatibility Complex (MHC) class I molecules, which present peptide fragments from within a cell to cytotoxic T cells as part of the adaptive immune system. B2M plays an essential role both in governing MHC class I molecule stability and in promoting antigen binding and presenting the antigen to CD3/TCR complex of CD8+ T cells.CIITA (Class II Transactivator, also known as Class II Major Histocompatibility Complex Transactivator) acts as a coactivator for MHC class II-specific gene expression. IFN-Ƴ induces CIITA gene expression via the JAK1 and STAT1 pathways. Defects in CIITA have been implicated in Bare Lymphocyte Syndrome (BLS), which is characterized by the absence of MHC class II transcription and severe immunodeficiencies.
Description: Both B2M (Beta-2-Microglobulin) and CIITA (Class II Transactivator) have been genetically removed from THP-1 cells using CRISPR/Cas9 genome editing.
Host Cell Line: THP-1, monocyte, suspension
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Supplied As: Each vial contains 2 x 106 cells in 1 ml of cell freezing medium (BPS Bioscience, #79796)
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-1
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