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Application: Study and screen compounds that inhibit the binding of TACI to BAFF for drug discovery in high throughput screening (HTS) applications.
Background: TACI (Transmembrane activator and CAML interactor), also known as tumor necrosis factor receptor superfamily member 13B (TNFRSF13B), is a lymphocyte-specific member of the TNF (Tumor necrosis factor) receptor superfamily, which regulates B and T cell function and humoral immunity. This receptor binds to ligands APRIL (A proliferation-inducing ligand) and BAFF (B-cell Activating Factor), two ligands that also bind to BCMA (B-cell maturation antigen) to induce B cell proliferation. TACI induces antibody responses and plasma cell differentiation, and it counteracts BAFF-driven B cell activation. Upon ligand binding, TACI interacts with CAML (calcium-modulator and cyclophilin ligand) to promote calcineurin-dependent activation of transcription factor NFAT (Nuclear Factor of Activated T cells). Although they do not directly cause disease, mutations in the TNFRSF13B gene predispose patients with CVID (common variable immunodeficiency) to autoimmune and immune cell proliferation disorders, such as SLE (systemic lupus erythematosus), lupus nephritis (LN) and RA (rheumatoid arthritis). The development of inhibitors targeting TACI:BAFF will open new therapeutic avenues.
Contraindications: This kit is compatible with up to 1% final DMSO concentration.
Description: The BAFF: TACI [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is an ELISA-based assay designed to measure the binding between BAFF (B-cell Activating Factor) and TACI (Transmembrane activator and CAML interactor, also known as tumor necrosis factor receptor superfamily member 13B (TNFRSF13B)) for screening and profiling applications. This kit comes with enough purified BAFF (amino acids 134-285) and biotin-labeled TACI (amino acids 23-166), streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions. Figure 1. BAFF: TACI [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit schematic. A 96-well plate is coated with BAFF protein. After coating and blocking, biotinylated TACI is added in an optimized assay buffer. Next, unbound biotinylated TACI is washed away, and the plate is incubated with streptavidin-HRP. After a final wash, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to TACI binding to BAFF. Need us to run inhibitor screens or profile your compounds against BAFF:TACI? Check out our Immunotherapy Screening Services.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Supplied As: This kit comes with enough purified BAFF (amino acids 134-285) and biotin-labeled TACI (amino acids 23-166), streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions.
Target: BAFF, TACI
Uniprot: Q9Y275 and O14836
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Smulski C., et al., 2017 Cell Reports 18(9): P2189-2202. Zeng L., et al., 2024 Journal of Autoimmunity 148: 103291.
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