Description: Bromodeoxyuridine (BrdU) is a synthetic nucleoside thymidine analog. It is commonly used to detect proliferation in cells. In cells cultured in the presence of BrdU, BrdU will incorporate into newly synthesized DNA during S-phase. Antibodies specific for BrdU can then be used to detect replicated DNA. This antibody is available purified, with BSA (0.2 mg/mL) or purified, BSA-free (1 mg/mL).
Product Origin: Animal - Mus musculus (mouse)
Conjugate: Purified, BSA-free
Concentration: 1 mg/mL
Storage buffer: PBS, no BSA, no azide
Clone: BRD469 BRD494 BRD.3
Immunogen: Bromodeoxyuridine (BrdU) conjugated to BSA or KLH
Antibody Reactivity: BrdU
Entrez Gene ID: Not Applicable
Z-Antibody Applications: IHC, FFPE (verified)
Verified AB Applications: IHC (FFPE) (verified)
Antibody Application Notes: Immunohistology formalin-fixed 0.5-1 ug/mL/Staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/mL PBS, 10 min at 37C/Immunofluorescence 0.5-1 ug/mL/Flow Cytometry 0.5-1 ug/million cells/Optimal dilution for a specific application should be determined by user
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