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Encompassing Amino Acids: CD33 1-16, CathepsinB 18-339
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 25 mM MES, pH 5.0, 10 µMsubstrate (Z-Leu-Arg-AMC), 10 min, roomtemperature. Prior to assay enzyme wasactivated with preincubation at 10 µg/ml in 25mM MES, pH 5.0, 5 mM DTT for 15 min at RT.
Description: Human cathepsin B (GenBank Accession No. NM_001908), CD33 signal peptide (a.a. 1-16) + CathepsinB (a.a. 18-339), with C-terminal His-tag, MW=43 kDa, expressed in FreeStyle 293-F cells.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Genbank: NM_001908
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal His-tag
Uniprot: P07858
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Tu, C. et al., Cancer Res. 2008 Nov 15;68(22):9147-56.
2. Hwang, JH. et al., Cancer Lett. 2009 Jan18;273(2):266-72.
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