CD106 / VCAM1(1.4C3), CF740 conjugate, 0.1mg/mL

Description: CD106 (also known as vascular cell adhesion molecule-1, or VCAM-1) is a member of the Ig superfamily of adhesion molecules. It is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on un-stimulated endothelial cells. It is also present on follicular and inter-follicular dendritic cells of lymph nodes, myoblasts, and some macrophages. CD106 serves as a ligand for leukocyte beta-1 integrin VLA-4 and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in various immunological and inflammatory responses.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product origin: Animal - Mus musculus (mouse), Bos taurus (bovine)

Conjugate: CF740

Concentration: 0.1 mg/mL

Storage buffer: PBS, 0.1% rBSA, 0.05% azide

Clone: 1.4C3

Immunogen: Stimulated human umbilical vein endothelial cells (HUVEC)

Antibody Reactivity: CD106/VCAM-1

References: Note: References for this clone sold by other suppliers may be listed for expected applications.

1. J Virol (1997) 71(11): 8522-8530. (WB)
2. J Immunol (1999) 162: 2990-2996. (IP; WB)
3. Cardiovasc Res (2007) 76(3): 494-505. (Flow, surface; cell-based ELISA)

Entrez Gene ID: 7412

Expected AB Applications: Flow (published for clone)/IP (published for clone)/WB (published for clone)

Z-Antibody Applications: Flow (published)/IHC, FFPE (verified)/IP (published)/WB (published)

Verified AB Applications: IHC (FFPE) (verified)

Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunofluorescence: 1-2 ug/mL/Immunohistology formalin-fixed 0.5-1 ug/mL/Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes/Flow Cytometry 0.5-1 ug/million cells/0.1 mL/Optimal dilution for a specific application should be determined by user

• SKU: BTMBNC740149-500
• Manufacturer: Biotium
• Manufacturer SKU: BNC740149-500
• Package Unit: 500 uL
• Quantity Unit: STK
• Reactivity: Human
• Clonality: Monoclonal
• Application: Immunoprecipitation, Western Blotting, Flow Cytometry, Immunohistochemistry
• Isotype: IgG1 kappa
• Host: Mouse
• Conjugate: Conjugated, CF740