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Applications: This kit is useful for screening for inhibitors of CD112 (PVRL2) binding to CD112R (PVRIG).
Background: CD112 (Poliovirus receptor-related 2, PVRL2), widely expressed on antigen-presenting cells and tumor cells, is the high affinity ligand of CD112R (Poliovirus receptor related immunoglobulin domain containing, PVRIG). CD112-CD112R interaction is a positive immune checkpoint that enhances human T cell response and it has emerged as an attractive therapeutic target for oncology. TIGIT (T cell immunoreceptor with Ig and ITIM domains) and CD226 (also called DNAM-1, DNAX Accessory Molecule-1) are other ligands of CD112.
Description: The CD112R:CD112[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD112R-CD112. The key to this kit is the high sensitivity of detection of biotin-labeled CD112 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CD112R is coated on a 96-well plate. Next, CD112 is incubated with CD112R on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.
Storage Stability: Up to 6 months from date of receipt, when stored as recommended.
Supplied As: This kit comes in a convenient 96-well format, with biotin-labeled CD112, purified CD112R, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.
Warnings: Avoid multiple freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Zhu, Y., et al. J. Exp. Med. 2016, 213(2):167-76
Torphy, R., et al. Int. J. Mol. Sci. 2017, 18(12): E2642
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