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Applications: The CD155/HEK293 Stable Cell Line functions as an antigen-presenting cell (APC) to stimulate TIGIT-specific signaling responses in lymphocytes, such as cell proliferation and cytokine release, or with a TIGIT reporter cell line (BPS Cat. #60538).
Background: CD155 is a transmembrane glycoprotein receptor belonging to theimmunoglobulin superfamily. It is highly expressed on dendritic cells, fibroblasts, endothelialcells, and multiple tumor cells including ovarian carcinoma, non-small cell lung, glioblastoma,and colorectal carcinoma. It has a high affinity for a T cell regulatory transmembrane surfaceprotein called TIGIT (T cell immunoglobulin and ITIM domains), and plays a key role in tumorcell invasion and migration. Interaction between CD155 and TIGIT causes immunosuppressiveeffects on CD4+ and CD8+ T cells, which hinders T cell proliferation and function. CD155 alsointeracts with regulatory receptors CD226 (expressed on natural killer (NK) cells, monocytesand CD4+ T cells) and CD96. While CD155-CD226 engagement activates NK cell cytotoxicityand T cell response, CD155-CD96 interaction inhibits NK cell function. Due to these opposingregulatory effects, blockade of CD155-TIGIT and CD155- CD226 interactions are attractivetherapeutic strategies in cancer immunotherapy and autoimmune diseases, respectively.
Description: Recombinant HEK293 cell line expressing full length human CD155, also knownas poliovirus receptor (PVR) or nectin-like molecule-5 (NECL-5). Expression is confirmed byWestern Blot.
Format: Aqueous solution containing DMSO
Host Cell Line: HEK293
Mycoplasma Testing: This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza, Cat.#LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set(Lonza, Cat. #LT07-518) was used as a positive control.
Storage Stability: Store in liquid nitrogen immediately upon receipt.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-2
References: 1. Yu, X., et.al. (2009) The Surface Protein TIGIT Suppresses T cell Activation by Promoting the Generation of Mature Immunoregulatory Dendritic Cells. Nat Immunol. 10: 48-57.
2. Bottino, C., et.al. (2003) Identification of PVR (CD155) and Nectin-2 (CD112) as Cell Surface Ligands for the Human DNAM-1 (CD226) Activating Molecule. J Exp Med. 198: 557-567.
3. Sloan, K.E., et.al. (2005) CD155/PVR Enhances Glioma Cell Dispersal by Regulating Adhesion Signaling and Focal Adhesion Dynamics. Cancer Res. 65: 10930-37.
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