CD25(143-13), 1mg/mL

Description: Recognizes a protein of 55 kDa, identified as CD25 (Workshop IV; Code A27). CD25 is expressed on activated T- and B-cells and activated monocytes/macrophages. With respect to lymphomas, CD25 is present on malignant cells of Hodgkin's disease, HTLV-1 adult T-cell leukemia, cutaneous T-cell lymphoma, and hair cell leukemia. Increased levels of soluble CD25 are observed in the leukemias/lymphomas and inflammatory/ autoimmune diseases. CD25 molecule alone appears to function as a low affinity receptor and associates with CD122 (IL-2R beta chain, p75) and CD132 (common gamma chain) to form the high affinity IL-2 receptor complex. CD25 antibodies detect three epitope regions, A, B and C. This MAb recognizes the epitope B, which is located at residue 3-104 of CD25 and can effectively block IL-2 binding to CD25.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Origin: Animal - Mus musculus (mouse)

Conjugate: Purified, BSA-free

Concentration: 1 mg/mL

Storage buffer: PBS, no BSA, no azide

Clone: 143-13

Immunogen: Stimulated human leukocytes

Antibody Reactivity: CD25/IL2RA

References: Note: References for this clone sold by other suppliers may be listed for expected applications.

1. Knapp W. et al. Leucocyte typing IV, p. 408- 411 and p. 1080, Oxford University Press, Oxford 1989.
2. Eur J Immunol (1991) 21: 1085-1088. (functional studies)
3. Tissue Antigens (1996) 48: 626-635. (Flow)
4. Gut (2006) 55(11): 1568-1574. (IHC, frozen)

Entrez Gene ID: 3559

Expected AB Applications: Flow, surface (published for clone)/Functional studies (published for clone)/IHC (frozen) (published for clone)

Z-Antibody Applications: Flow, surface (published)/Functional studies (published)/IHC, frozen (published)

Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunofluorescence: 0.5-1 ug/mL/Flow Cytometry 0.5-1 ug/million cells/0.1 mL/Optimal dilution for a specific application should be determined by user

• SKU: BTMBNUM0297-50
• Manufacturer: Biotium
• Manufacturer SKU: BNUM0297-50
• Package Unit: 50 µl
• Quantity Unit: STK
• Reactivity: Human
• Clonality: Monoclonal
• Application: Immunohistochemistry (frozen), Flow Cytometry, Functional Studies
• Isotype: IgG1 kappa
• Host: Mouse
• Conjugate: Unconjugated