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Encompassing Amino Acids: 21-182
Applications: Useful as a positive control for Western blotting.
Description: Mouse secreted CD27, Fc fusion protein, with C-terminal Avi-Tag, also known as Tumor Necrosis Factor Receptor Superfamily Member 7, TNFRSF7, and T14, GenBank Accession No. NM_001033126, a.a. 21-182 expressed in a HEK293 cell expression system. MW = 49 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Genbank: NM_001033126
Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.
Tags: C-terminal Fc fusion (IgG1) Avi-Tag
Biosafety Level: Not applicable (BSL-1)
References: 1.Hendricks, J. et al., Nat. Immunol. 2000; 1: 433-440.2.Ruprecht, C.R. et al., J. Exp. Med. 2005; 201(11): 1793-1803.
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