Description: Recognizes a 67 kDa glycoprotein, which is identified as CD33 (HLDA IV; WS Code M-505). CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immuno-receptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. CD33 is expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. It is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product Origin: Animal - Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Conjugate: CF405S
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% BSA, 0.05% azide
Clone: WM53
Immunogen: Human AML cells
Antibody Reactivity: CD33
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
- Immunity (2002) 17(3), 289-301. (Flow)
- J Leukicyte Biol (2006) 79(1), 46-58. (Flow, IP)
- OncoImmunology, 7:4, DOI: 10.1080/2162402X.2017.1397248 (immunohistochemistry)
- Glycobiology (2011) 21(6), 757-770 (epitope mapping)
Entrez Gene ID: 945
Expected AB Applications: Flow, surface (published for clone)/IHC (FFPE) (published for clone)
Z-Antibody Applications: Flow, surface (published)/IHC, FFPE (published)
Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunofluorescence: 0.5-1 ug/mL/Flow Cytometry 0.5-1 ug/million cells/0.1 mL
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