Description: This antibody recognizes a protein of 30 kDa, identified as CD70. It is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T- and B-lymphocytes. It induces proliferation of co-stimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin synthesis. This MAb blocks the interaction between CD27 and CD70, and has been shown toinhibit T cell proliferation induced by dendritic cells.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: BU69
Immunogen: Human WM-1 (Waldenstrom s macroglobulinemia) cell line
Antibody Reactivity: CD70
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
- Lymphology (2003) 36: 74-83. (Flow)
- J Immunol (2006) 176:7726-7735. (functional studies)
- Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1137-1138.
Entrez Gene ID: 970
Expected AB Applications: Flow, surface (published for clone)/Functional studies (published for clone)
Z-Antibody Applications: Flow, surface (published)/Functional studies (published)
Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunoprecipitation: 0.5-1 ug/500 ug protein lysate/Immunofluorescence: 0.5-1 ug/mL/Immunohistology (frozen) 0.5-1 ug/mL/Flow Cytometry 0.5-1 ug/million cells/0.1 mL/ELISA For coating, order Ab without BSA/Inhibits T cell proliferation induced by dendritic cells/Optimal dilution for a specific application should be determined by user
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