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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: CDK7 gene is a member of the cyclin-dependent protein kinase family that is important regulators of cell cycle progression (1). CDK7 forms a trimeric complex with cyclin H and MAT1, which functions as a CDK-activating kinase (CAK). CDK7 is an essential component of the transcription factor TFIIH that is involved in transcription initiation and DNA repair. CDK7 is thought to serve as a direct link between the regulation of transcription and the cell cycle (2).
Description: Complex of recombinant human CDK7, Cyclin H1 and MNAT1, full length, with N-terminal His-tags (CDK7 and MNAT1) and C-terminal His-tag (CyclinH1) , (GenBank Accession Nos. NM_001799, NM_001239, and NM_002431 respectively), co-expressed in Sf9 insect cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 50 mM sodium phosphate pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, 25% glycerol
Genbank: NM_001799, NM_001239, NM_002431
Host Cell Line: Sf9 cells
Purity: ≥80%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag (CDK7 and MNAT1), C-terminal His-tag (CyclinH1)
Uniprot: CDK7: P50613; CyclinH1: P51946; MNAT1: P51948
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Fisher, R. P.;A novel cyclin associates with MO15/CDK7 to form the CDK-activating kinase. Cell 78: 713-724, 1994.
2. Larochelle, S. Requirements for Cdk7 in the assembly of Cdk1/cyclin B and activation of Cdk2 revealed by chemical genetics in human cells. Molec. Cell 25: 839-850, 2007.
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