The PeliKine compact human IL-6 ELISA Kit is the optimal tool for sensitive detection of the pro-inflammatory cytokine Interleukin 6 (IL-6) released by human monocytes during exposure to endotoxins or pyrogens in the Monocyte Activation Test (MAT). IL-6 is a mediator of inflammatory responses and is involved in the development of fever.
The PeliKine compact human IL-6 kit is a "sandwich-type" of enzyme immunoassay in which a monoclonal antibody is pre-coated to polystyrene microtiter wells. The target cytokine, present in a measured volume of sample or standard, is bound by the antibody on the microtiter plate. Non-bound material is then removed by washing. Subsequently, a biotinylated antibody is added. This antibody binds to the cytokine-antibody complex present in the microtiter well. Excess biotinylated antibody is removed by washing, followed by addition of horseradish peroxidase (HRP) conjugated streptavidine, which binds onto the biotinylated side of the cytokine sandwich. After removal of non-bound HRP conjugate by washing, substrate solution is added to the wells. A colored product is formed in proportion to the amount of cytokine present in the sample or standard. After the reaction has been terminated by the addition of a stop solution, absorbance is measured in an absorbance reader at 450 nm.
English
Deutsch
