Products from BPS Bioscience require a minimum order value above 400€
Application: Use as a negative control when testing CD38-directed therapies in A549 cells.Study the phenotype resulting from CD38 knockout.
Background: CD38 (Cluster of Differentiation 38, Cyclic ADP-Ribose Hydrolase 1, or ADPRC1) is a glycoprotein and ectoenzyme playing an important role in regulating intracellular calcium. CD38 is a highly attractive target antigen for immunotherapy because it is highly expressed on multiple myeloma (MM) cells, and at relatively low levels on normal lymphoid and myeloid cells. Expression of CD38 has also been associated with HIV infection, leukemia, and type II diabetes mellitus (T2D). In 2015, the FDA approved daratumumab (Darzalex), a breakthrough therapy monoclonal antibody targeting CD38, for the treatment of MM. CD38 has become an interesting target for the development of CAR (chimeric receptor antigen)-T cells, which are T cell product engineered by retroviral transduction to express a fully human CD38-specific CAR. The level of upregulation of CD38 in lung cancer patients correlates with patient survival, with its enzymatic activity leading to increased cell migration, proliferation and tumor progression. Future development and studies will open new therapeutic avenues for CD38-related diseases, such as lung cancer.
Description: CD38 Knockout A549 Cell Line is a A549 lung cancer cell line in which human CD38 (Cluster of Differentiation 38) has been genetically removed using CRISPR/Cas9 genome editing with a lentivirus encoding the CRISPR/Cas9 gene and sgRNA (single guide RNA) targeting human CD38.
Host Cell Line: A549 is a human lung alveolar vessel carcinoma cell line. Adherent epithelial cells.
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Supplied As: Each vial contains ˃1 x 106 cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)
Target: CD38
Uniprot: P28907
Unit Definition: Cell Line
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-1
References: Cui Q., et al., 2021 J Hematol Oncol. 14(1):82.Gao L., et al., 2021 Cell Death Dis. 12(7):680.
English
Deutsch
