Firefly Luciferase A549 Cell Line

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Application: Use as target cells in CAR-T or CAR-NK co-culture killing assays.In vitro and in vivo bioluminescence imaging.

Background: A549 cells were isolated from the lung tissue of a lung cancer patient. This cell line is the most commonly used human non-small cell lung cancer (NSCLC) cell line for both basic cancer research and drug discovery. Firefly luciferase has been used as a sensitive reporter to study a wide range of biological responses. It was first cloned from the North American Photinus pyralis and catalyzes the oxidation of D-luciferin, in the presence of ATP and magnesium, emitting yellow light. This reaction has a high quantum yield, and both luciferase and luciferin have low toxicity. These characteristics contributed to Firefly luciferase becoming a commonly used tool. The use of firefly luciferase as reporter allows for easy readouts.

Description: Firefly Luciferase A549 Cell Line is a lung cancer A549 cell line that expresses the firefly luciferase reporter driven by an EF1a promoter. This cell line has been generated by transduction with Firefly Luciferase Lentivirus (BPS Bioscience #78740-H).This cell line has been validated in co-culture assays.

Host Cell Line: A549 is a human lung alveolar vessel carcinoma cell line. Adherent epithelial cells.

Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.

Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Supplied As: Each vial contains ≥1 x 106 cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Unit Definition: Cell Line

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: BSL-1

• SKU: BPS82495
• Manufacturer: BPS Bioscience
• Manufacturer SKU: 82495
• Package Unit: 2 vials
• Quantity Unit: PAK
• Host: Human