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Application: Useful for screening for modulators of IL-11 mediated signaling pathways.
Background: Interleukin-11 (IL-11) is multifunctional cytokine that belongs to the IL-6 family of cytokines, which commonly activate glycoprotein 130 (gp130), a signal transducing receptor protein. Initially recognized for its role in hematopoiesis, further research has indicated that IL-11 may play an important role in the pathogenesis of fibro-inflammatory diseases. IL-11 is produced in tissues throughout the body with the primary cellular producer being stromal fibroblasts found in the lungs, liver, heart, and GI (gastrointestinal) tract. Signaling is initiated when IL-11 binds to its specific membrane bound receptor, IL-11 receptor alpha (IL-11Rα or IL-11Ra), followed by binding to its co-receptor gp130 (IL-6ST, IL-6 cytokine family signal transducer) and subsequent dimerization with another gp130 molecule. This results in the activation of Janus kinases (JAKs), predominately JAK1 and JAK2, prompting phosphorylation and activation of signal transducer and activator of transcription (STAT) molecules STAT1 and STAT3. While typically undetectable in healthy individuals, elevated expression of IL-11 has been identified in the tissues of patients with idiopathic pulmonary fibrosis, kidney fibrosis, and cardiac fibrosis. The development of therapeutics targeting IL-11 is an active area of research with clinical trials currently ongoing for the treatment of fibrotic diseases.
Description: The IL-11 (Interleukin-11) Responsive Luciferase Reporter HEK293 Line is a HEK293 cell line engineered to express both human IL-11Ra (IL-11 receptor alpha) (NM_001142784.3) and human IL-6ST (IL-6 cytokine family signal transducer, also known as gp130) (NM_002184.4) separated by a self-cleaving P2A peptide. The construct was delivered by lentiviral transduction of STAT3 Luciferase Reporter HEK293 Cell Line (#79800-P), which express a firefly luciferase reporter driven by STAT3 response elements located upstream of the minimal TATA promoter. After activation by IL-11, the endogenous transcription factor STAT3 binds to the response elements, inducing transcription of the luciferase reporter gene.This cell line has been validated to respond to IL-11. Figure 1: Illustration of the mechanism of action in the IL-11 Responsive Luciferase Reporter HEK293 Cell Line.Interested in screening and profiling inhibitors, blocking antibodies, or activators of IL-11 signaling without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.
Host Cell Line: HEK293, Human Embryonic Kidney, epithelial-like cells, adherent.
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Supplied As: Each vial contains ≥1 x 106 cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)
Target: IL-11, IL-11R
Uniprot: P20809, Q14626
Unit Definition: Cell Line
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-2
References: Campbell C., et al., 2001 Am J Pathol. 158(1):25-32.Cook S., et al., 2020 Annu Rev Med. 71:263-276.Fung K., et al., 2022 Cytokine. 149:155750. Jones S., et al., 2018 Nat Rev Immunol. 18(12):773-789.Lokau J., et al., 2021 Biochim Biophys Acta Mol Cell Res. 1869(1):119135.Putoczki T., et al., 2013 Cancer Cell. 24(2): 257-71.
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